anti- ALCAM antibody

Host

Rabbit

Uniprot ID

Q13740

Form

liquid

Purification

Immunogen affinity purified

Purity

>= 95%

Clonality

polyclonal

Clone ID

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts(PubMed:7760007, PubMed:15496415, PubMed:15048703, PubMed:16352806, PubMed:23169771, PubMed:24945728). Promotes T-cell activation and proliferation via its interactions with CD6(PubMed:15048703, PubMed:16352806, PubMed:24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6(PubMed:15294938, PubMed:16352806). Mediates homotypic interactions with cells that express ALCAM(PubMed:15496415, PubMed:16352806). Required for normal hematopoietic stem cell engraftment in the bone marrow(PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction(PubMed:23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions(PubMed:15496415, PubMed:23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation(By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons(By similarity). Isoform 3: Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.

Immunogen

activated leukocyte cell adhesion molecule

Synonyms

ALCAM, CD166, CD166 antigen, FLJ38514, MEMD

Observed MW

65 kDa, observed 110 kDa

Reactivity

Human

Tested Application

ELISA, IHC, WB

Recommended dilution

WB: 1:200-1:2000; IHC: 1:20-1:200

Gene ID

214

Research Area

Stem Cells, Immunology, Signal Transduction, Developmental biology