anti- Acetylated tubulin(Lys40) antibody

Host

Mouse

Uniprot ID

Q71U36

Form

liquid

Purification

Protein A+G purification

Purity

? 95% as determined by SDS-PAGE

Clonality

monoclonal

Clone ID

6A10

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin.

Immunogen

tubulin, alpha 1a

Synonyms

Acetylated alpha tubulin, Acetylated tubulin, acetylated-a-tubulin, acetyl-a-tubulin, alpha tubulin, Alpha tubulin 3, tubulin,TUBA1A

Observed MW

52 kDa

Reactivity

Human, Mouse, Rat, Dog

Tested Application

ELISA, WB, IHC, IF

Recommended dilution

WB: 1:500-1:5000; IF: 1:20-1:200; IHC: 1:20-1:200

Gene ID

7846

Research Area

Neuroscience, Cell Division and Proliferation, Metabolism