anti- 6*His, His-Tag antibody

Host

Mouse

Uniprot ID

Form

liquid

Purification

Protein A+G purification

Purity

? 95% as determined by SDS-PAGE

Clonality

monoclonal

Clone ID

1C4

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

Immunogen

Synonyms

6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag, HISTIDINE TAGGED

Observed MW

Reactivity

Recombinant protein

Tested Application

ELISA, WB, IP, IF

Recommended dilution

WB: 1:1000-1:10000; IP: 1:1000-1:10000; IF: 1:20-1:100

Gene ID

Research Area