AIFM1 Antibody

Size

100ul

Clone Number

4B2

Aliases

Apoptosis-inducing factor 1, mitochondrial (EC 1.1.1.-) (Programmed cell death protein 8), AIFM1, AIF PDCD8

Immunogen Species

Homo sapiens (Human)

UniProt ID

O95831

Immunogen

A synthesized peptide derived from human AIF

Raised in

Species Reactivity

Human, Rat

Tested Applications

ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200

Background

Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

Clonality

Monoclonal

Isotype

Rabbit IgG

Purification Method

Affinity-chromatography

Conjugate

Non-conjugated

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

AIFM1

Research Areas

Epigenetics and Nuclear Signaling; Cancer; Cell biology; Metabolism