ACTB Monoclonal Antibody

Size

Clone Number

Aliases

ACTB; BRWS1; PS1TP5BP1

Immunogen Species

Human

UniProt ID

P60709

Immunogen

Recombinant Protein

Raised in

Mouse

Species Reactivity

Human,Rat,Mouse,Monkey,Dog

Tested Applications

ELISA,WB;Recommended dilution:WB:1:500-1:5000

Background

Beta-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against Beta-actin are useful as loading controls for Western Blotting. However it should be noted that levels of Beta-actin may not be stable in certain cells. For example, expression of Beta-actin in adipose tissue is very low and therefore Beta-actin should not be used as loading control for these tissues.

Clonality

Monoclonal

Isotype

IgG1

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

ACTB

Research Areas

Isotype/Loading Controls;Tags & Cell Markers;Signal transduction