CLIA Kit for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

20.6-15,000pg/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 7.3pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Assay length

2h, 40min

Method

Double-antibody Sandwich

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) level in the sample or standard.;

Alternative Names

PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1

Item Name

Ubiquitin Carboxyl Terminal Hydrolase L1

Research Area

Enzyme & Kinase;

Reference