ELISA Kit for Apelin 17 (AP17)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

24.69-2,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 9.14pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Apelin 17 (AP17) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Apelin 17 (AP17) and unlabeled Apelin 17 (AP17) (Standards or samples) with the pre-coated antibody specific to Apelin 17 (AP17). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Apelin 17 (AP17) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Apelin 17 (AP17) in the sample.

Alternative Names

APLN17

Item Name

Apelin 17

Research Area

Reference

Serum concentrations of apelin-17 isoform vary in accordance to blood pressure categories in individuals with obesity class 3;