ELISA Kit for Microphthalmia Associated Transcription Factor (MITF)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.059ng/mL

Sample type

tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microphthalmia Associated Transcription Factor (MITF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microphthalmia Associated Transcription Factor (MITF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microphthalmia Associated Transcription Factor (MITF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microphthalmia Associated Transcription Factor (MITF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

WS2A; MI; bHLHe32; Homolog Of Mouse Microphthalmia; Class E basic helix-loop-helix protein 32

Item Name

Microphthalmia Associated Transcription Factor

Research Area

Signal transduction;

Reference

Melanogenesis and antioxidant defense system in normal human melanocytes cultured in the presence of chlorpromazine;Effect of thioridazine on antioxidant status of HEMn-DP melanocytes;FLUPHENAZINE AND PERPHENAZINE IMPACT ON MELANOGENESIS AND ANTIOXIDANT ENZYMES ACTIVITY IN NORMAL HUMAN MELANOCYTES;Prochlorperazine interaction with melanin and melanocytes;