CLIA Kit for Protein Kinase C Epsilon (PKCe)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.14-100ng/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.07ng/mL

Sample type

Tissue homogenates and other biological fluids

Assay length

2h, 40min

Method

Double-antibody Sandwich

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Epsilon (PKCe). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Protein Kinase C Epsilon (PKCe). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Protein Kinase C Epsilon (PKCe) level in the sample or standard.;

Alternative Names

PKC-E; PRKCE; PRKC-E; NPKC-Epsilon

Item Name

Protein Kinase C Epsilon

Research Area

Signal transduction;Enzyme & Kinase;Apoptosis;Endocrinology;Cardiovascular biology;

Reference