CLIA Kit for Macrophage Migration Inhibitory Factor (MIF)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

13.7-10,000pg/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 5.9pg/mL

Sample type

Serum, plasma, tissue homogenates and other biological fluids

Assay length

2h, 40min

Method

Double-antibody Sandwich

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Macrophage Migration Inhibitory Factor (MIF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Macrophage Migration Inhibitory Factor (MIF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Macrophage Migration Inhibitory Factor (MIF) level in the sample or standard.;

Alternative Names

GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

Item Name

Macrophage Migration Inhibitory Factor

Research Area

Cytokine;Infection immunity;Rheumatology;Autoimmunity;

Reference