ELISA Kit for Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.312-20ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.113ng/mL

Sample type

Tissue homogenates and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

DHC1; DHC1a; DNCH1; DNCL; DNECL; DYHC; Dnchc1; HL-3; p22; Dynein heavy chain, cytosolic; Cytoplasmic dynein heavy chain 1; Cytoplasmic dynein 1 heavy chain 1

Item Name

Dynein, Cytoplasmic 1, Heavy Chain 1

Research Area

Metabolic pathway;

Reference