CLIA Kit for Ghrelin (GHRL)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

39.06-10,000pg/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Chicken (Gallus)

Sensitivity

The minimum detectable dose of this kit is typically less than 17.31pg/mL

Sample type

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Ghrelin (GHRL). A competitive inhibition reaction is launched between biotin labeled Ghrelin (GHRL) and unlabeled Ghrelin (GHRL) (Standards or samples) with the pre-coated antibody specific to Ghrelin (GHRL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ghrelin (GHRL) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Ghrelin (GHRL) level in the sample or standard.

Alternative Names

MTLRP; Growth Hormone-Releasing Peptide

Item Name

Ghrelin

Research Area

Signal transduction;Metabolic pathway;Endocrinology;Gastroenterology;Hormone metabolism;

Reference