ELISA Kit for Transferrin (TF)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

78.12-5000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Equus caballus; Equine (Horse)

Sensitivity

The minimum detectable dose of this kit is typically less than 34ng/mL

Sample type

Serum, plasma and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transferrin (TF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transferrin (TF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transferrin (TF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transferrin (TF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

TRF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin

Item Name

Transferrin

Research Area

Tumor immunity;Infection immunity;Immune molecule;

Reference