ELISA Kit for Gastrin Releasing Peptide (GRP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.69pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gastrin Releasing Peptide (GRP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gastrin Releasing Peptide (GRP) and unlabeled Gastrin Releasing Peptide (GRP) (Standards or samples) with the pre-coated antibody specific to Gastrin Releasing Peptide (GRP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gastrin Releasing Peptide (GRP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gastrin Releasing Peptide (GRP) in the sample.

Alternative Names

BN; GRP10; Bombesin; Neuromedin C

Item Name

Gastrin Releasing Peptide

Research Area

Endocrinology;

Reference

Relationships among plasma granzyme B level, pruritus and dermatitis in patients with atopicdermatitis.;