ELISA Kit for Cyclin Dependent Kinase 6 (CDK6)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.053ng/mL

Sample type

Tissue homogenates and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase 6 (CDK6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase 6 (CDK6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase 6 (CDK6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase 6 (CDK6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

PLSTIRE; Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE

Item Name

Cyclin Dependent Kinase 6

Research Area

Enzyme & Kinase;Metabolic pathway;Tumor immunity;Developmental science;

Reference

The nanoparticulate Quillaja saponin KGI exerts anti-proliferative effects by down-regulation of cell cycle molecules in U937 and HL-61 human leukemia cells;