ELISA Kit for Glucose Transporter 1 (GLUT1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

1.25-80ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.51ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose Transporter 1 (GLUT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose Transporter 1 (GLUT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose Transporter 1 (GLUT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose Transporter 1 (GLUT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

SLC2A1; SLC2-A1; GLUT; Solute Carrier Family 2 Member 1,Facilitated Glucose Transporter; Glucose transporter type 1, erythrocyte/brain; HepG2 glucose transporter

Item Name

Glucose Transporter 1

Research Area

Signal transduction;Metabolic pathway;Endocrinology;

Reference

Oral formulation of DPP-4 inhibitor plus Quercetin improves metabolic homeostasis in type 1 diabetic rats;