ELISA Kit for Apelin 13 (AP13)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

74.07-6000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 26.38pg/mL

Sample type

Serum, plasma and other biological fluids.

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Apelin 13 (AP13) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Apelin 13 (AP13) and unlabeled Apelin 13 (AP13) (Standards or samples) with the pre-coated antibody specific to Apelin 13 (AP13). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Apelin 13 (AP13) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Apelin 13 (AP13) in the sample.

Alternative Names

APLN13

Item Name

Apelin 13

Research Area

Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Gastroenterology;

Reference

Is short-term exercise a therapeutic tool for improvement of cardioprotection against DOX-induced cardiotoxicity? An experimental controlled protocol in rats.;Individual and concomitant effects of cardioprotective programs on cardiac apelinergic system and oxidative state in L-NAME-induced hypertension.;Improvement of Kidney Apelin and Apelin Receptor in Nitro-L-Arginine-Methyl Ester Induced Rats;