ELISA Kit for Fatty Acid Binding Protein 4 (FABP4)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

31.2-2,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 13.8pg/mL

Sample type

serum, plasma, tissue homogenates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 4 (FABP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Binding Protein 4 (FABP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Binding Protein 4 (FABP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Binding Protein 4 (FABP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

A-FABP; AFABP; AP2; Fatty Acid Binding Protein 4, Adipocyte; Adipocyte Protein 2; Adipocyte-type fatty acid-binding protein

Item Name

Fatty Acid Binding Protein 4

Research Area

Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;

Reference

Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice;Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytes;