ELISA Kit for Retinoid X Receptor Alpha (RXRa)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.067ng/mL

Sample type

Tissue homogenates and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoid X Receptor Alpha (RXRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoid X Receptor Alpha (RXRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoid X Receptor Alpha (RXRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinoid X Receptor Alpha (RXRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

NR2B1; RXR-A; NR2-B1; Nuclear Receptor Subfamily 2,Group B,Member 1; Retinoic acid receptor RXR-alpha

Item Name

Retinoid X Receptor Alpha

Research Area

Signal transduction;Metabolic pathway;Endocrinology;Hormone metabolism;

Reference

The Crucial Involvement of Retinoid X Receptors in DDE Neurotoxicity;