ELISA Kit for Forkhead Box P3 (FOXP3)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.312-20ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.113ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Forkhead Box P3 (FOXP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Forkhead Box P3 (FOXP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Forkhead Box P3 (FOXP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Forkhead Box P3 (FOXP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

FOX-P3; AIID; DIETER; IPEX; JM2; PIDX; XPID; SCURFIN; Forkhead Box Protein P3; Immune Dysregulation,Polyendocrinopathy,Enteropathy,X-Linked

Item Name

Forkhead Box P3

Research Area

Signal transduction;Apoptosis;Tumor immunity;Infection immunity;Immune molecule;

Reference