ELISA Kit for Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

6.25-100ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 2.66ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

PEPCK1; PEPCKC; Cytosolic Phosphoenolpyruvate Carboxykinase; Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Item Name

Phosphoenolpyruvate Carboxykinase 1, Soluble

Research Area

Enzyme & Kinase;Metabolic pathway;Endocrinology;

Reference

In utero exposure to the endocrine disruptor di-(2-ethylhexyl) phthalate induces long-term changes in gene expression in the adult male adrenal gland;