ELISA Kit for Activin A Receptor Type II A (ACVR2A)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.057ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activin A Receptor Type II A (ACVR2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activin A Receptor Type II A (ACVR2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activin A Receptor Type II A (ACVR2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activin A Receptor Type II A (ACVR2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

ACVR2-A; ACTRII; ACTR-II; ACVR2

Item Name

Activin A Receptor Type II A

Research Area

Signal transduction;Endocrinology;Hormone metabolism;

Reference

Detection of Sotatercept (ACE-011) in human serum by SAR-PAGE and western single blotting.;活化素A 在原发性干燥综合征患者中的表达分析研究;