ELISA Kit for SET Domain Containing Protein 7 (SETD7)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.054ng/mL

Sample type

Tissue homogenates and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SET Domain Containing Protein 7 (SETD7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SET Domain Containing Protein 7 (SETD7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SET Domain Containing Protein 7 (SETD7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SET Domain Containing Protein 7 (SETD7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

SET7; SET7/9; Set9; KMT7; Lysine N-methyltransferase 7; Histone H3-K4 methyltransferase SETD7; Histone-lysine N-methyltransferase SETD7

Item Name

SET Domain Containing Protein 7

Research Area

Signal transduction;

Reference