ELISA Kit for Complement Receptor 2 (CD21)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

1.56-100ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.57ng/mL

Sample type

Serum, plasma and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Receptor 2 (CD21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Receptor 2 (CD21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Receptor 2 (CD21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Receptor 2 (CD21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CR2; C3DR; Complement Component(3d/Epstein Barr Virus)Receptor 2; Complement C3d receptor; Epstein-Barr virus receptor; EBV receptor

Item Name

Complement Receptor 2

Research Area

Signal transduction;CD & Adhesion molecule;Infection immunity;Immune molecule;

Reference

Complement receptor 2 is up regulated in the spinal cord following nerve root injury and modulates the spinal cord response;