ELISA Kit for Parathyroid Hormone Receptor 1 (PTHR1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.312-20ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.127ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Parathyroid Hormone Receptor 1 (PTHR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Parathyroid Hormone Receptor 1 (PTHR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Parathyroid Hormone Receptor 1 (PTHR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Parathyroid Hormone Receptor 1 (PTHR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

PTH1R; Parathyroid hormone/parathyroid hormone-related peptide receptor; PTH/PTHrP type I receptor

Item Name

Parathyroid Hormone Receptor 1

Research Area

Endocrinology;

Reference

PLXNA2 identified as a candidate gene by genome-wide association analysis for mandibular prognathism in human chondrocytes;