ELISA Kit for Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

123.5-10,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 48.7ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) and unlabeled Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) (Standards or samples) with the pre-coated antibody specific to Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) in the sample.

Alternative Names

Item Name

Nicotinic Acid Adenine Dinucleotide Phosphate

Research Area

Reference