ELISA Kit for Chitinase-3-like Protein 1 (CHI3L1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

3.12-200ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.28ng/mL

Sample type

Serum, plasma and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chitinase-3-like Protein 1 (CHI3L1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chitinase-3-like Protein 1 (CHI3L1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chitinase-3-like Protein 1 (CHI3L1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chitinase-3-like Protein 1 (CHI3L1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

GP39; CHI3-L1; YKL40; Chondrex; Chitinase 3-Like 1; Chondrocyte Protein YKL40; 39 kDa synovial protein

Item Name

Chitinase-3-like Protein 1

Research Area

Metabolic pathway;Infection immunity;Bone metabolism;Rheumatology;

Reference

YKL-40 is a local marker for inflammation in patients with pseudoexfoliation syndrome;