ELISA Kit for Nuclear Receptor Subfamily 0,Group B,Member 2 (NR0B2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.054ng/mL

Sample type

Tissue homogenates and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Receptor Subfamily 0,Group B,Member 2 (NR0B2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Receptor Subfamily 0,Group B,Member 2 (NR0B2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Receptor Subfamily 0,Group B,Member 2 (NR0B2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Receptor Subfamily 0,Group B,Member 2 (NR0B2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

SHP; SHP1; Small Heterodimer Partner; Orphan nuclear receptor SHP

Item Name

Nuclear Receptor Subfamily 0,Group B,Member 2

Research Area

Signal transduction;Endocrinology;Developmental science;Hormone metabolism;

Reference

Raspberry pomace alters cecal microbial activity and reduces secondary bile acids in rats fed a high-fat diet.;