ELISA Kit for Indoleamine-2,3-Dioxygenase 2 (IDO2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

31.25-2000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 12.9pg/mL

Sample type

Serum, plasma, tissue homogenates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Indoleamine-2,3-Dioxygenase 2 (IDO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Indoleamine-2,3-Dioxygenase 2 (IDO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Indoleamine-2,3-Dioxygenase 2 (IDO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Indoleamine-2,3-Dioxygenase 2 (IDO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

INDOL1; Indoleamine-Pyrrole 2,3 Dioxygenase-Like 1; Indoleamine 2,3-dioxygenase-like protein 1; Indoleamine-pyrrole 2,3-dioxygenase-like protein 1

Item Name

Indoleamine-2,3-Dioxygenase 2

Research Area

Enzyme & Kinase;

Reference