ELISA Kit for B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.312-20ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.118ng/mL

Sample type

tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

B-raf 1; BRAF1; RAFB1; V-Raf Murine Sarcoma Viral Oncogene Homolog B1

Item Name

B-Raf Proto Oncogene Serine/Threonine Protein Kinase

Research Area

Enzyme & Kinase;

Reference