CLIA Kit for Ubiquitin Conjugating Enzyme E2L3 (UBE2L3)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

13.7-10,000pg/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 5.7pg/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

2h, 40min

Method

Double-antibody Sandwich

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Conjugating Enzyme E2L3 (UBE2L3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ubiquitin Conjugating Enzyme E2L3 (UBE2L3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) level in the sample or standard.;

Alternative Names

L-UBC; E2-F1; UBCH7; UbcM4; Ubiquitin carrier protein L3; Ubiquitin-conjugating enzyme E2-F1; Ubiquitin-protein ligase L3

Item Name

Ubiquitin Conjugating Enzyme E2L3

Research Area

Enzyme & Kinase;

Reference