CLIA Kit for Choline Acetyltransferase (ChAT)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

13.7-10,000pg/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 5.9pg/mL

Sample type

Tissue homogenates and other biological fluids

Assay length

2h, 40min

Method

Double-antibody Sandwich

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Choline Acetyltransferase (ChAT). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Choline Acetyltransferase (ChAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Choline Acetyltransferase (ChAT) level in the sample or standard.;

Alternative Names

CMS1A; CMS1A2; CHOACTase; Choline acetylase

Item Name

Choline Acetyltransferase

Research Area

Enzyme & Kinase;Metabolic pathway;Cardiovascular biology;Neuro science;

Reference

Trans-resveratrol Inhibits Tau Phosphorylation in the Brains of Control and Cadmium Chloride-Treated Rats by Activating PP2A and PI3K/Akt Induced-Inhibition of …;