ELISA Kit for Cortisone (Cor)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

2.47-200ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.93ng/mL

Sample type

Serum, plasma, urine and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cortisone (Cor) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cortisone (Cor) and unlabeled Cortisone (Cor) (Standards or samples) with the pre-coated antibody specific to Cortisone (Cor). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cortisone (Cor) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cortisone (Cor) in the sample.

Alternative Names

17OHCS; 17-OHCS; 17-Hydroxy-11-Dehydrocorticosterone; 17-Hydroxycorticosteroid

Item Name

Cortisone

Research Area

Reference