ELISA Kit for Copeptin (CPP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

15.6-1000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 6.2pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Copeptin (CPP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Copeptin (CPP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Copeptin (CPP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Copeptin (CPP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

C-Terminal Provasopressin; Vasopressin-Neurophysin 2-Copeptin

Item Name

Copeptin

Research Area

Reference

Copeptin: May it be a novel biomarker for insulin theraphy in diabetes? An animal study.;Role of enteroendocrine L‐cells in arginine vasopressin‐mediated inhibition of colonic anion secretion;Copeptin level in the early prediction of cardiorenal syndrome in rats;和肽素水平对大鼠心肾综合征的早期预测价值;