ELISA Kit for Apelin (APLN)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

98.77-8000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 35.3pg/mL

Sample type

Serum, plasma and other biological fluids.

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Apelin (APLN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Apelin (APLN) and unlabeled Apelin (APLN) (Standards or samples) with the pre-coated antibody specific to Apelin (APLN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Apelin (APLN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Apelin (APLN) in the sample.

Alternative Names

XNPEP2; APEL; APJ endogenous ligand

Item Name

Apelin

Research Area

Endocrinology;

Reference

CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes;TIMP3 interplays with apelin to regulate cardiovascular metabolism in hypercholesterolemic mice;