ELISA Kit for Salusin Beta (SALb)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

4.94-400pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.94pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Salusin Beta (SALb) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Salusin Beta (SALb) and unlabeled Salusin Beta (SALb) (Standards or samples) with the pre-coated antibody specific to Salusin Beta (SALb). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Salusin Beta (SALb) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Salusin Beta (SALb) in the sample.

Alternative Names

Item Name

Salusin Beta

Research Area

Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;

Reference

Superoxide anions involved in sympathoexcitation and pressor effects of salusin-β in paraventricular nucleus in hypertensive rats;Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats.;Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy;Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleus;