ELISA Kit for WAP Four Disulfide Core Domain Protein 5 (WFDC5)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

78.12-5000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 35pg/mL

Sample type

Serum, plasma and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WAP Four Disulfide Core Domain Protein 5 (WFDC5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WAP Four Disulfide Core Domain Protein 5 (WFDC5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WAP Four Disulfide Core Domain Protein 5 (WFDC5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WAP Four Disulfide Core Domain Protein 5 (WFDC5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

WAP1; Putative protease inhibitor WAP1; p53-responsive gene 5 protein

Item Name

WAP Four Disulfide Core Domain Protein 5

Research Area

Signal transduction;

Reference