ELISA Kit for Interleukin 8 Receptor Beta (IL8Rb)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

31.2-2,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 13.1pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 8 Receptor Beta (IL8Rb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 8 Receptor Beta (IL8Rb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 8 Receptor Beta (IL8Rb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 8 Receptor Beta (IL8Rb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CD182; CDw128b; IL8R-B; IL8-RB; CMKAR2; CXCR2; IL8R2; C-X-C chemokine receptor type 2; GRO/MGSA receptor; High affinity interleukin-8 receptor B

Item Name

Interleukin 8 Receptor Beta

Research Area

Signal transduction;Tumor immunity;Infection immunity;Dermatology;

Reference

Role of ceramide synthase 2 in G-CSF signaling and G-CSF-R translocation into detergent-resistant membranes;