ELISA Kit for Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

78.12-5000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 32pg/mL

Sample type

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

MyHC-peri; MyHC-pn; MyHC-perinatal; Myosin heavy chain, skeletal muscle, perinatal

Item Name

Myosin Heavy Chain 8, Skeletal Muscle, Perinatal

Research Area

Metabolic pathway;

Reference