ELISA Kit for Cysteine Conjugate Beta Lyase, Cytoplasmic (CCbL1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

78.12-5000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 27pg/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Conjugate Beta Lyase, Cytoplasmic (CCbL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Conjugate Beta Lyase, Cytoplasmic (CCbL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Conjugate Beta Lyase, Cytoplasmic (CCbL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteine Conjugate Beta Lyase, Cytoplasmic (CCbL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

KATI; GTK; Cysteine-S-conjugate beta-lyase; Glutamine-phenylpyruvate transaminase; Kyneurenine Aminotransferase; Kynurenine Oxoglutarate Transaminase

Item Name

Cysteine Conjugate Beta Lyase, Cytoplasmic

Research Area

Enzyme & Kinase;

Reference