ELISA Kit for Cardiolipin (CL)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

3.9-1,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.5ng/mL

Sample type

Tissue homogenates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cardiolipin (CL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cardiolipin (CL) and unlabeled Cardiolipin (CL) (Standards or samples) with the pre-coated antibody specific to Cardiolipin (CL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cardiolipin (CL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cardiolipin (CL) in the sample.

Alternative Names

1,3-Bis(sn-3-Phosphatidyl)-Sn-Glycerol

Item Name

Cardiolipin

Research Area

Reference