ELISA Kit for Major Histocompatibility Complex Class I A (MHCA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.312-20ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.111ng/mL

Sample type

Serum, plasma, tissue homogenates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I A (MHCA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I A (MHCA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I A (MHCA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Major Histocompatibility Complex Class I A (MHCA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

HLA-A; HLAA; MHC-A; Leukocyte Antigen A; HLA class I histocompatibility antigen, A-31 alpha chain

Item Name

Major Histocompatibility Complex Class I A

Research Area

Immune molecule;

Reference

Two-peaked increase of serum myosin heavy chain-α after triathlon suggests heart muscle cell death;Aerobic capacity predicts skeletal but not cardiac muscle damage after a full distance Ironman triathlon-the Iron (wo) man-study;