ELISA Kit for Protein Kinase C Epsilon (PKCe)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

1.56-100ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.58ng/mL

Sample type

tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Epsilon (PKCe). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase C Epsilon (PKCe). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase C Epsilon (PKCe), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase C Epsilon (PKCe) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

PKC-E; PRKCE; PRKC-E; NPKC-Epsilon

Item Name

Protein Kinase C Epsilon

Research Area

Signal transduction;Enzyme & Kinase;Apoptosis;Endocrinology;Cardiovascular biology;

Reference

Effect of single and repeated heat stress on chemical signals of heat shock response cascade in the rat's heart.;