ELISA Kit for Vasoactive Intestinal Peptide (VIP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

6.17-500pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Cavia (Guinea pig )

Sensitivity

The minimum detectable dose of this kit is typically less than 2.52pg/mL

Sample type

Serum, plasma and other biological fluids.

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vasoactive Intestinal Peptide (VIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vasoactive Intestinal Peptide (VIP) and unlabeled Vasoactive Intestinal Peptide (VIP) (Standards or samples) with the pre-coated antibody specific to Vasoactive Intestinal Peptide (VIP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vasoactive Intestinal Peptide (VIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vasoactive Intestinal Peptide (VIP) in the sample.

Alternative Names

PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27

Item Name

Vasoactive Intestinal Peptide

Research Area

Endocrinology;Gastroenterology;Hormone metabolism;

Reference

Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide;Effects of sphincter of Oddi motility on the formation of cholesterol gallstones;