ELISA Kit for Cholecystokinin 8 (CCK8)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Cavia (Guinea pig )

Sensitivity

The minimum detectable dose of this kit is typically less than 5.28pg/mL

Sample type

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cholecystokinin 8 (CCK8) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cholecystokinin 8 (CCK8) and unlabeled Cholecystokinin 8 (CCK8) (Standards or samples) with the pre-coated antibody specific to Cholecystokinin 8 (CCK8). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cholecystokinin 8 (CCK8) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cholecystokinin 8 (CCK8) in the sample.

Alternative Names

Sincalide; Cholecystokinin 8, Octapeptide

Item Name

Cholecystokinin 8

Research Area

Endocrinology;Gastroenterology;Hormone metabolism;

Reference

Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide;