CLIA Kit for Angiotensin I (AngI)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

7.81-2,000pg/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 2.92pg/mL

Sample type

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Angiotensin I (AngI). A competitive inhibition reaction is launched between biotin labeled Angiotensin I (AngI) and unlabeled Angiotensin I (AngI) (Standards or samples) with the pre-coated antibody specific to Angiotensin I (AngI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin I (AngI) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Angiotensin I (AngI) level in the sample or standard.

Alternative Names

Ang-I; Angiotensin-1

Item Name

Angiotensin I

Research Area

Endocrinology;Cardiovascular biology;Hepatology;Hormone metabolism;

Reference