ELISA Kit for 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

24.69-2,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 9.11pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) and unlabeled 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) (Standards or samples) with the pre-coated antibody specific to 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) in the sample.

Alternative Names

8-EpiPGF2-α; 8epi-PGF2a

Item Name

8-Epi Prostaglandin F2 Alpha

Research Area

Endocrinology;Pulmonology;Hepatology;Hormone metabolism;

Reference

Relationships between glucose fluctuations and oxidative stress in short duration type 2 diabetes treated with sensor-augmented insulin pump.;Hormonal and metabolic indicators before and after farrowing in sows affected with postpartum dysgalactia syndrome;