ELISA Kit for Peroxisome Proliferator Activated Receptor Gamma (PPARg)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.78-50ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Oryctolagus cuniculus (Rabbit)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.30ng/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Gamma (PPARg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Gamma (PPARg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Gamma (PPARg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxisome Proliferator Activated Receptor Gamma (PPARg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3

Item Name

Peroxisome Proliferator Activated Receptor Gamma

Research Area

Metabolic pathway;Endocrinology;Cardiovascular biology;Developmental science;Bone metabolism;

Reference

Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone;Establishment of a rabbit model to study the influence of advanced glycation end productsaccumulation on osteoarthritis and the protective effect of pioglitazone.;