SKU:ABCC25840
ELISA Kit for Platelet Derived Growth Factor D (PDGFD)
ELISA Kit for Platelet Derived Growth Factor D (PDGFD)
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Detection range
0.312-20ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Organism species
Rattus norvegicus (Rat)
Sensitivity
The minimum detectable dose of this kit is typically less than 0.125ng/mL
Sample type
Serum, plasma and other biological fluids.
Assay length
3h
Method
Double-antibody Sandwich
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor D (PDGFD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor D (PDGFD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor D (PDGFD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Derived Growth Factor D (PDGFD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Alternative Names
PDGF-D; IEGF; SCDGF-B; Spinal Cord Derived Growth Factor B; Iris-expressed growth factor; Platelet-derived growth factor D, receptor-binding form/latent form
Item Name
Platelet Derived Growth Factor D
Research Area
Cytokine;