ELISA Kit for Gastrin (GT)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Cavia (Guinea pig )

Sensitivity

The minimum detectable dose of this kit is typically less than 4.35pg/mL

Sample type

Serum, plasma and other biological fluids.

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gastrin (GT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gastrin (GT) and unlabeled Gastrin (GT) (Standards or samples) with the pre-coated antibody specific to Gastrin (GT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gastrin (GT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gastrin (GT) in the sample.

Alternative Names

GAST; GAS; Gastrin component I; Big gastrin; Gastrin component II; Gastrin component III

Item Name

Gastrin

Research Area

Metabolic pathway;Endocrinology;Gastroenterology;Hormone metabolism;

Reference

Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide;Effects of sphincter of Oddi motility on the formation of cholesterol gallstones;